| # | Name | Position | Height | Weight |
|---|---|---|---|---|
| 10 | Jermaine Agee | DB | 5'11" | 184 |
| 99 | Oyeka Alexander | DL | 6'2" | 307 |
| 92 | Roosevelt Andrews III | DL | 6'2" | 264 |
| 55 | Ayden Annarino | OL | 6'3" | 295 |
| 71 | Lucas Barnes | OL | 6'8" | 290 |
| 8 | Koy Beasley | DB | 5'10" | 187 |
| 48 | Chase Becker | TE | 6'4" | 228 |
| 2 | Devin Bell | DL | 6'1" | 250 |
| 10 | Lynel Billups-Williams | WR | 5'9" | 179 |
| 28 | Andrew Bjorson | WR | 6'1" | 195 |
| 1 | Eli Blakey | S | 6'2" | 214 |
| 27 | Gino Blasini | DB | 6'2" | 205 |
| 89 | Brayden Boeing | TE | 6'5" | 230 |
| 25 | Mitch Bolden | TE | 6'0" | 202 |
| 15 | Harrison Brewster | LB | 6'1" | 210 |
| 6 | Jordan Brunson | RB | 6'0" | 233 |
| 87 | Noam Butbul | TE | 6'0" | 225 |
| 56 | Mitchell Butler | OL | 6'4" | 275 |
| 55 | Malachi Clark | DL | 6'1" | 303 |
| 21 | Toney Coleman Jr. | DB | 6'2" | 193 |
| 28 | Leo Colombi | DB | 6'3" | 207 |
| 4 | Deion Colzie | WR | 6'4" | 211 |
| 26 | Eli Coppess | LB | 6'1" | 213 |
| 90 | Donovan Davis | DL | 6'1" | 302 |
| 35 | Tyler Dikos | LB | 6'1" | 225 |
| 31 | Dom Dzioban | K | 6'1" | 208 |
| 11 | Noah Ehrlich | QB | 6'1" | 195 |
| 5 | Luke Evans | DB | 6'2" | 186 |
| 22 | Steven Favazzo | DB | 5'11" | 201 |
| 1 | Dequan Finn | QB | 6'2" | 206 |
| 50 | Jayden Fuller | LB | 6'0" | 234 |
| 21 | Tito Glass Jr. | RB | 6'2" | 238 |
| 97 | William Goodvine III | DL | 6'1" | 278 |
| 13 | Thomas Gotkowski | QB | 6'1" | 193 |
| 63 | Jameil Hamm | DL | 6'4" | 235 |
| 9 | Javon Hammonds | DL | 6'4" | 240 |
| 12 | Henry Hesson | QB | 6'2" | 197 |
| 36 | Carter Holden | K | 6'4" | 206 |
| 12 | Corban Hondru | LB | 6'1" | 227 |
| 54 | Aiden Howard | OL | 6'5" | 286 |
| 93 | Logan Howell | DL | 6'4" | 312 |
| 17 | Braylon Isom | WR | 6'3" | 206 |
| 22 | Bai Jobe | DL | 6'4" | 240 |
| 14 | D'Shawntae Jones | RB | 5'10" | 245 |
| 83 | Eric Karner | TE | 6'5" | 230 |
| 88 | Brody Kosin | TE | 6'6" | 234 |
| 19 | Jonah Kroenung | QB | 6'0" | 195 |
| 73 | Brandon Lawhorn Moore | OL | 6'6" | 308 |
| 44 | Grant Leeper | TE | 6'5" | 248 |
| 81 | Brendan Loftus | TE | 6'7" | 215 |
| 61 | Andrew Lowry | OL | 6'8" | 310 |
| 49 | Anthony Ludington | DL | 6'4" | 230 |
| 94 | Josh Lukusa | DL | 6'4" | 257 |
| 43 | Grant Lyons | LB | 6'3" | 238 |
| 72 | Kris Manu | OL | 6'3" | 274 |
| 17 | Kaleb Martin | DB | 6'2" | 173 |
| 58 | Gray Maultsby | LS | 6'5" | 240 |
| 3 | Malcolm McCain | LB | 6'1" | 241 |
| 37 | Vito McConnell | LB | 6'3" | 218 |
| 16 | Christian McKinney | LB | 6'0" | 216 |
| 38 | Kellan McLaughlin | K | 5'10" | 177 |
| 23 | Oscar McWood | LB | 6'1" | 216 |
| 14 | Khory Moss | DB | 5'11" | 188 |
| 46 | Luke Myers | LB | 6'1" | 212 |
| 62 | Logan Neu | DL | 6'3" | 246 |
| 67 | Carson Noonan | DL | 6'3" | 270 |
| 96 | Carmine Orozco | DL | 6'4" | 275 |
| 82 | Paolo Papalia | WR | 5'9" | 185 |
| 80 | Michael Parrish | WR | 6'2" | 190 |
| 8 | Kam Perry | WR | 5'9" | 168 |
| 22 | Jackson Pons | DB | 6'2" | 195 |
| 59 | Hudson Powell | LS | 6'3" | 221 |
| 53 | Malik Ray | LB | 5'11" | 230 |
| 0 | Keith Reynolds | WR | 5'9" | 180 |
| 47 | Brach Rice | LB | 6'3" | 241 |
| 30 | Josh Ringer | RB | 6'1" | 203 |
| 15 | Wilson Roberts | WR | 6'1" | 195 |
| 76 | Gavin Rohrs | OL | 6'4" | 319 |
| 11 | Isaiah Rollins | DB | 6'0" | 175 |
| 5 | Cordale Russell | WR | 6'4" | 210 |
| 79 | Jacob Schorsch | OL | 6'6" | 306 |
| 32 | James Schott | DL | 6'4" | 242 |
| 86 | Brian Shane | TE | 6'3" | 228 |
| 39 | Eli Shereshovech IV | DL | 6'5" | 280 |
| 2 | Brady Simmons | WR | 6'1" | 173 |
| 16 | Ryan Sims | WR | 6'3" | 217 |
| 51 | Eric Smith | OL | 6'6" | 299 |
| 19 | Jerome Smith | DB | 6'2" | 180 |
| 74 | Greg Smith Jr. | OL | 6'5" | 308 |
| 32 | Pierse Stainton | P | 6'1" | 190 |
| 79 | Jonathan Stangl | OL | 6'8" | 315 |
| 75 | Anthony Tanaka | OL | 6'4" | 295 |
| 95 | Aariz Taylor | DL | 6'4" | 230 |
| 20 | Ian Tepas | WR | 6'0" | 200 |
| 78 | Drew Terrill | OL | 6'6" | 280 |
| 18 | Antwon Thomas | WR | 5'10" | 171 |
| 9 | Kenny Tracy | RB | 5'10" | 223 |
| 20 | Adam Trick | LB | 6'4" | 242 |
| 18 | Brock Uihlein | LB | 6'2" | 225 |
| 77 | Austin Uke | OL | 6'3" | 315 |
| 6 | Adrian Walker Jr. | DB | 5'10" | 175 |
| 52 | Nasir Washington | DL | 6'4" | 269 |
| 84 | Cole Weaver | WR | 6'5" | 220 |
| 69 | Marcus White | OL | 6'3" | 295 |
| 91 | Sam Williams | DL | 6'2" | 236 |
| 3 | Darion Williamson | WR | 6'3" | 202 |
| 4 | Kameron Wilson | DL | 6'2" | 250 |
| 13 | Desmon Yharbrough | DB | 6'3" | 195 |
| 24 | Mychal Yharbrough | DB | 6'2" | 199 |
| 85 | Trey Zvonar | TE | 6'3" | 220 |
