| # | Name | Position | Height | Weight |
|---|---|---|---|---|
| 50 | Mitchell Agude | LB | - | - |
| 15 | Maurice Alexander | WR | - | - |
| 34 | Alex Anzalone | LB | 6'3" | 238 |
| 74 | Kayode Awosika | G | 6'5" | 305 |
| 17 | Michael Badgley | PK | 5'10" | 188 |
| 55 | Derrick Barnes | LB | 6'1" | 245 |
| - | Mathieu Betts | LB | 6'3" | 250 |
| 32 | Brian Branch | S | - | - |
| 46 | Jack Campbell | LB | - | - |
| 79 | John Cominsky | DE | 6'5" | 285 |
| 47 | Scott Daly | LS | 6'1" | 245 |
| - | Marcus Davenport | LB | 6'6" | 265 |
| - | Carlton Davis | CB | 6'1" | 206 |
| 68 | Taylor Decker | OT | 6'7" | 318 |
| 30 | Khalil Dorsey | CB | 5'8" | 185 |
| 66 | Matt Farniok | G | 6'6" | 335 |
| 84 | Daurice Fountain | WR | 6'2" | 210 |
| 3 | Jack Fox | P | 6'2" | 218 |
| - | Jake Funk | RB | 5'10" | 203 |
| 76 | Connor Galvin | OT | - | - |
| 26 | Jahmyr Gibbs | RB | - | - |
| 24 | Steven Gilmore | CB | - | - |
| 60 | Graham Glasgow | G | 6'6" | 315 |
| 16 | Jared Goff | QB | 6'4" | 217 |
| 80 | Antoine Green | WR | - | - |
| 12 | Hendon Hooker | QB | - | - |
| 41 | James Houston | LB | 6'1" | 233 |
| 97 | Aidan Hutchinson | DE | 6'6" | 278 |
| 38 | Justin Jackson | RB | 6'0" | 200 |
| - | Craig James | CB | 5'10" | 195 |
| 39 | Jermar Jefferson | RB | 5'10" | 214 |
| 40 | Brandon Joseph | S | - | - |
| 31 | Kerby Joseph | S | 6'1" | 200 |
| 85 | Tom Kennedy | WR | 5'10" | 195 |
| 28 | Zonovan Knight | RB | 6'0" | 197 |
| 87 | Sam LaPorta | TE | - | - |
| 98 | Brodric Martin | DT | - | - |
| 54 | Alim McNeill | DT | 6'2" | 315 |
| 6 | Ifeatu Melifonwu | S | 6'3" | 200 |
| 82 | James Mitchell | TE | 6'3" | 252 |
| 5 | David Montgomery | RB | 5'11" | 224 |
| 48 | Zach Morton | DE | - | - |
| 4 | Emmanuel Moseley | CB | 5'11" | 195 |
| - | Netane Muti | G | 6'3" | 307 |
| 62 | Michael Niese | G | - | - |
| 91 | Levi Onwuzurike | DE | 6'3" | 287 |
| 93 | Josh Paschal | DE | 6'3" | 274 |
| 19 | Donovan Peoples-Jones | WR | 6'2" | 204 |
| 77 | Frank Ragnow | C | 6'5" | 310 |
| 11 | Kalif Raymond | WR | 5'8" | 180 |
| - | D.J. Reader | DT | 6'3" | 335 |
| 42 | Jalen Reeves-Maybin | LB | 6'1" | 226 |
| 13 | Craig Reynolds | RB | 5'11" | 216 |
| - | Amik Robertson | CB | - | - |
| 44 | Malcolm Rodriguez | LB | 6'0" | 205 |
| 58 | Penei Sewell | OT | 6'6" | 330 |
| 70 | Dan Skipper | OT | 6'9" | 330 |
| 90 | Chris Smith | DT | - | - |
| - | Tre'Quan Smith | WR | 6'1" | 210 |
| 75 | Colby Sorsdal | OT | - | - |
| 14 | Amon-Ra St. Brown | WR | - | - |
| 10 | Nate Sudfeld | QB | 6'6" | 230 |
| 29 | Kindle Vildor | CB | 5'11" | 189 |
| 9 | Jameson Williams | WR | 6'2" | 188 |
| 89 | Brock Wright | TE | 6'4" | 246 |
| - | Kevin Zeitler | G | 6'4" | 340 |
| 84 | Shane Zylstra | TE | - | - |
