| # | Name | Position | Height | Weight |
|---|---|---|---|---|
| 17 | Josh Allen | QB | 6'5" | 237 |
| 70 | Alec Anderson | OT | 6'5" | 300 |
| 51 | Eli Ankou | DT | 6'3" | 325 |
| 2 | Tyler Bass | PK | 5'10" | 183 |
| 47 | Christian Benford | CB | - | - |
| 43 | Terrel Bernard | LB | 6'1" | 222 |
| 32 | Kyron Brown | CB | 6'1" | 195 |
| 79 | Spencer Brown | OT | - | - |
| 6 | Shane Buechele | QB | 6'1" | 207 |
| 77 | Will Clapp | C | 6'5" | 311 |
| 96 | Kameron Cline | DE | 6'4" | 283 |
| 71 | La'el Collins | OT | 6'4" | 320 |
| 4 | James Cook | RB | 5'11" | 190 |
| 84 | Zach Davidson | TE | - | - |
| 73 | Dion Dawkins | OT | 6'5" | 320 |
| 31 | Rasul Douglas | CB | 6'2" | 209 |
| 72 | Tommy Doyle | OT | 6'8" | 318 |
| 76 | David Edwards | G | 6'6" | 308 |
| 21 | Mike Edwards | S | 5'10" | 205 |
| 5 | Kaiir Elam | CB | 6'2" | 187 |
| 57 | A.J. Epenesa | DE | 6'6" | 260 |
| 37 | Darrynton Evans | RB | 5'10" | 203 |
| 69 | Reid Ferguson | LS | 6'2" | 235 |
| 41 | Reggie Gilliam | FB | 6'0" | 244 |
| 75 | Richard Gouraige | OT | - | - |
| 15 | Matt Haack | P | 6'0" | 205 |
| 19 | KJ Hamler | WR | 5'9" | 178 |
| 3 | Damar Hamlin | S | 6'1" | 195 |
| 13 | Mack Hollins | WR | 6'4" | 221 |
| 46 | Ja'Marcus Ingram | CB | 6'1" | 185 |
| 87 | Andy Isabella | WR | 5'9" | 188 |
| 63 | Kevin Jarvis | G | 6'4" | 307 |
| 98 | Austin Johnson | DT | 6'4" | 314 |
| 7 | Taron Johnson | CB | 5'11" | 192 |
| 26 | Ty Johnson | RB | 5'10" | 210 |
| 59 | Kingsley Jonathan | DE | 6'3" | 251 |
| 92 | DaQuan Jones | DT | 6'4" | 320 |
| 86 | Dalton Kincaid | TE | - | - |
| 88 | Dawson Knox | TE | 6'4" | 254 |
| 39 | Cam Lewis | CB | 5'9" | 183 |
| 8 | Sam Martin | P | 6'1" | 211 |
| 66 | Connor McGovern | G | 6'5" | 318 |
| 83 | Tre' McKitty | TE | 6'5" | 245 |
| 58 | Matt Milano | LB | 6'0" | 223 |
| 40 | Von Miller | LB | 6'3" | 250 |
| 85 | Quintin Morris | TE | 6'4" | 228 |
| 52 | Nicholas Morrow | LB | 6'0" | 216 |
| 91 | Ed Oliver | DT | 6'1" | 287 |
| 20 | Taylor Rapp | S | 6'0" | 208 |
| 50 | Greg Rousseau | DE | 6'6" | 260 |
| 1 | Curtis Samuel | WR | 5'11" | 195 |
| 10 | Khalil Shakir | WR | 6'0" | 185 |
| 80 | Tyrell Shavers | WR | - | - |
| 18 | Justin Shorter | WR | - | - |
| 54 | Baylon Spector | LB | 6'1" | 230 |
| 89 | Bryan Thompson | WR | - | - |
| 99 | Casey Toohill | DE | 6'5" | 254 |
| 64 | O'Cyrus Torrence | G | - | - |
| 11 | Mitch Trubisky | QB | 6'2" | 220 |
| 74 | Ryan Van Demark | OT | 6'6" | 293 |
| 93 | DeShawn Williams | DT | 6'1" | 295 |
| 42 | Dorian Williams | LB | - | - |
| 36 | Kendall Williamson | S | - | - |
